Basecalling, alignment, assembly and deconvolution of Sanger Chromatogram trace files
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Updated
Jul 24, 2026 - C++
Basecalling, alignment, assembly and deconvolution of Sanger Chromatogram trace files
In-silico PCR, primer design and padlock design for in-situ sequencing
sangerseq_viewer is a python package to automatically visualize Sanger sequencing results and the corresponding annotated sequence map.
Indigo: SNV and InDel Discovery in Chromatogram traces obtained from Sanger sequencing of PCR products
An AI-native molecular biology workbench for Claude Science: Claude opens sequence files and prepares analyses on request; you annotate, align, and review them in an interactive workspace.
In-silico PCR amplification on complete genomes
Patching references via trace assemblies
A tool to batch-generate optimal DNA primers for Sanger sequencing. Output .xlsx file is directly compatible with IDT Oligo Entry.
A local API-based biological engine that uses Tracy for sequence alignment and VEP for variant annotation. Built with Python for computational biology and bioinformatics analysis.
Historical archive: Python 2.7-era tool for checking Sanger sequencing results against references. Not maintained.
Comprehensive Sanger sequence analysis and clinical reporting tool. Identifies SNVs and Indels precisely while keeping your genetic data secure locally.
GEAR home page
Automated DNA barcoding pipeline: chromatogram → phylogeny → species ID. Zero coding required. Built for ENTM201L @ UC Riverside. Docker + GitHub Classroom ready.
World-class, high-performance web viewer for Sanger sequencing traces (.ab1/.scf): chromatogram rendering, base calls, quality scores. Client-side, hosted on GitHub Pages.
シーケンス簡易アセンブリスクリプト Simple Sequence Assembly Script
Training offered to Students of ACE Mali. Intro to NGS and Sanger Sequencing including the technology, sequencers and their applicability to differen research questions.
Extract and distribute ab1 files from Sanger DNA sequencing to folders of their respective owners.
A complete visual study guide for interpreting Sanger sequencing chromatograms. Learn to differentiate technical artifacts from real biological variants.
A pipeline to process ab1 files through to functional fungal guilds
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